Verify that the antibody is definitely able to detect the antigen in the species by checking data sheets and reference material Potential Cause: Primary and secondary antibody mismatch Raise the ...

GE Healthcare Life Sciences recently launched the Amersham WB system for quantitative SDS-PAGE and Western blotting of proteins using fluorescence detection. The system was standardized for the entire ...

Western blotting remains a cornerstone method for the detection and quantification of proteins from complex biological samples. This technique has evolved from its early qualitative roots to a more ...

Western blot detection methods each have their own advantages and disadvantages. Choosing the right one can be a difficult decision. The basic protocol of the western blot – also referred to as the ...

It takes time to get the hang of new immunodetection methods, and during this learning period you may well see your share of failed experiments. Christiana Binkley, a PhD student in cellular and ...

In this editorial, Kenneth Oh overviews the two main normalization methods used to quantify western blots and discusses common errors to avoid. What is western blot normalization? Kenneth Oh is the ...

Meet the trusted FluorChem TM Western blot imaging systems. Simply load your sample, choose a protocol, and hit expose. Load less protein, use smaller amounts of your precious antibodies, and never ...

Mariella is an assistant editor at The Scientist. She has a background in neuroscience, and her work has appeared in Drug Discovery News and Massive Science. View full profile. Learn about our ...

A classic laboratory technique, western blotting, has been used by researchers for decades, and it is nearly obsolete. "Biologists currently are stuck in a time warp—they basically use the same tools ...

We used western blot (WB) to compare the IgG response to varicella-zoster virus (VZV) after chickenpox (CP), zoster, and administration of a live attenuated varicella vaccine (LAVV). After CP, 13 of ...