Western blotting is a very useful and widely adopted lab technique, but the traditional procedure can be long and tedious. Researchers can assess only whether a blot image is captured at the end of ...

Verify that the antibody is definitely able to detect the antigen in the species by checking data sheets and reference material Potential Cause: Primary and secondary antibody mismatch Raise the ...

Western blotting can often be more trouble than expected. While SDS-PAGE and western blotting protocols are straightforward, there are many critical decision points that lead to success or failure. In ...